Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing

摘要

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

主权项

1. A method for extracting biomolecules from a portion of cells from a cell sample on a carrier, wherein the cell sample includes a plurality of cells, the method comprising:
contacting a stand-off portion of the carrier to a substrate, wherein the stand-off portion is configured to maintain a distance between the substrate and a transfer film, wherein a cell sample is included on the substrate; transferring a portion of cells selected for analysis from the cell sample included on the substrate to the transfer film, wherein the transfer film is included on the carrier; mating the carrier to an extraction device, wherein the extraction device is configured to remove biomolecules from the portion of cells selected for analysis, wherein the extraction device comprises:
a carrier-receiving portion comprising a plurality of flanges, wherein the carrier-receiving portion comprises an inner surface, wherein the carrier-receiving portion further includes a landing portion having a landing surface, the landing surface defining an inner opening, anda conduit interconnected to the carrier-receiving portion, the conduit extending between a first opening on the carrier-receiving portion and a second opening at a second end, wherein the carrier-receiving portion is adapted to receive the carrier and to form a reservoir by the carrier contacting the landing portion, wherein the carrier mating with the carrier-receiving portion closes the inner opening to seal the inner opening to prevent fluid flow through a bottom surface; deflecting the flanges by passing a at least a portion of the carrier between the flanges so as secure the carrier to the extraction device in a compression-fit engagement; forming the reservoir with the transfer film by contacting the stand-off portion to the landing surface, wherein the stand-off portion and landing surface are configured so that the stand-off portion is not included in the reservoir; providing fluid into the reservoir to extract biomolecules from the portion of cells from the transfer film; and removing the fluid from the reservoir.