| 摘要 |
<p>TITLE: DETECTION OF CERVICAL PRE-CANCERS USING A NOVEL SANDWICH ELISA APPLICANT NAME: CANCER INSTITUTE ABSTRACT The present invention shall disclose an accurate and reliable method for the early detection of cervical cancer with minimal false result. The method involves collecting the cervical smear samples and detecting the presence of p16 in the collected samples by sandwich ELISA using novel monoclonal antibodies developed against recombinant p16 protein and detecting the presence of cytokeratin using direct ELISA to obtain p160D and cytokeratin OD. From the values of both the ODs the results were ascertained for the accurate presence or absence of cervical cancer. The invention also discloses the method of developing novel monoclonal antibodies against recombinant p16 protein for use in sandwich ELISA and thereby reliably detecting p16 even at 2 picogram level. The invention also discloses a kit for accurate, reliable and early detection of cervical cancer in a subject with minimal false results and avoiding the need for trained Pathologists or CytoPathologists or Cyto-Technologists.</p> |
