BINDING-INDUCED FORMATION OF DNA THREE-WAY JUNCTIONS FROM NON-DNA TARGETS

摘要

A method of detecting a non-DNA target includes the use of a first nucleic acid motif linked to a first affinity ligand which binds specifically to the target and having a first toehold domain and a first binding domain, and a second nucleic acid motif linked to a second affinity ligand which binds specifically to the target and having a second binding domain, wherein the first and second binding domains are complementary to each other. Upon contact with the target, the first and second nucleic acid motifs bind to form a target-ligand complex. The formation of the complex causes displacement of an output nucleic acid motif. This method may be used with detectable beacons in an imaging or diagnostic method, and particularly in a point of care diagnostic method.

主权项

1. A method of detecting a non-DNA target, comprising:
(a) providing a first nucleic acid motif linked to a first affinity ligand which binds specifically to the target and having a first toehold domain and a first binding domain, and a second nucleic acid motif linked to a second affinity ligand which binds specifically to the target and having a second binding domain, wherein the first and second binding domains are complementary to each other; (b) contacting the target with the first and second nucleic acid motifs to form a target-ligand complex, wherein the formation of the complex causes displacement of an output nucleic acid motif.