NOVEL BIOMARKERS FOR NON-ALCOHOLIC FATTY LIVER DISEASE, AND METHODS FOR DETECTING NON-ALCOHOLIC FATTY LIVER DISEASE BY USING SUCH BIOMARKERS

摘要

The present invention aims to present methods to detect nonalcoholic fatty liver disease including nonalcoholic steatohepatitis by using a protein or its partial peptide that differs in presence or absence, or in quantity between healthy human subjects and patients with nonalcoholic fatty liver disease or nonalcoholic steatohepatitis or between patients with fatty liver and nonalcoholic steatohepatitis and further aims to present biomarkers comprising said protein and said partial peptide to be used to detect nonalcoholic fatty liver disease including nonalcoholic steatohepatitis. Specifically, 35 kDa protein fragment consisting of amino acid sequence expressed by Sequence No. 2 and its partial peptide consisting of amino acid sequence expressed by Sequence No. 3 (including its glycated form) of inter-alpha-trypsin inhibitor heavy chain H4 precursor consisting of amino acid sequence expressed by Sequence No. 1 could be used as biomarkers to detect nonalcoholic fatty liver disease including nonalcoholic steatohepatitis.

主权项

1. A method for detection and treatment of nonalcoholic fatty liver disease in a patient, the method comprising:
detecting at least one biological marker by the binding of at least antibody or aptamer in biological material from a patient wherein the biological marker is selected from one or more of (i) protein of SEQ ID NO: 2; and (ii) protein fragment of not less than 5 contiguous amino acid residues of SEQ ID NO: 2; determining the presence of nonalcoholic fatty liver disease in the patient by the amount of at the least one biomarker detected in the biological material from the patient; and treating the patient with diet or drug therapy if the presence of nonalcoholic fatty liver disease has been determined.