SYSTEM AND METHODS FOR NUCLEIC ACID SEQUENCING OF SINGLE MOLECULES BY POLYMERASE SYNTHESIS

摘要

This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.

主权项

1. An apparatus for sequencing a plurality of target nucleic acid molecules comprising:
a support comprising a patterned array of bioadhesive features, whereby the bioadhesive features are formed at a size whereby just one molecule is immobilized at each feature in the patterned array, wherein each one molecule comprises a polymerase enzyme or a target nucleic acid, and each one molecule comprises a portion of a complex comprising a nucleic acid polymerizing enzyme, a target nucleic acid, and a primer which complements a region of the target nucleic acid, wherein the nucleic acid polymerizing enzyme is complexed with the target nucleic acid and the primer; reaction reagents in contact with the support including more than one type of labeled nucleotide triphosphate (NTP), wherein each type of NTP is differently labeled with a detectable label under conditions whereby polymerase extension products are formed; and a detector that individually detects labeled NTPs while the polymerase extension products are formed.