SHORTENED PURIFICATION PROCESS FOR PRODUCTION OF CAPSULAR STREPTOCOCCUS PNEUMONIAE POLYSACCHARIDES

摘要

PROBLEM TO BE SOLVED: To provide a shortened process for producing a substantially purified solution containing capsular polysaccharides from a cellular Streptococcus pneumoniae lysate broth.SOLUTION: A process for producing capsular polysaccharides comprises: ultrafiltering and diafiltering a clarified S. pneumoniae lysate, followed by pH adjustment to less than 4.5, preferably about 3.5, thereby precipitating protein in the solution; and performing further ultrafiltration and diafiltration, and acidification to a pH of less than 4.5, followed by filtration using activated carbon.