ADIPOSE TISSUE CENTRIFUGE AND METHOD OF USE

摘要

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

主权项

1. A centrifuge for processing a biologic mixture and selectively concentrating constituents of the biologic mixture, the constituents having differing specific gravities and being stratifiable in a centrifugal field produced by said centrifuge, said centrifuge comprising:
a. a processing assembly comprising:
i. an inner chamber arranged to receive the biologic mixture, and having a central longitudinal axis about which said inner chamber is arranged to be rotated, said inner chamber comprising a tapered tubular member having a sidewall and a base, said tapered tubular member having at least one extrusion hole extending radially therethrough and located at a first position along the central longitudinal axis;ii. an outer chamber arranged to receive a biologic mixture from said inner chamber, said outer chamber arranged coaxially upon said central longitudinal axis of said inner chamber and around said inner chamber, said outer chamber having a sidewall and a dish and being arranged to rotate about said central longitudinal axis;iii. at least one cutting element positioned between a portion of said inner chamber and said outer chamber, said at least one cutting element being arranged to remain stationary relative to the rotation of said inner and outer chambers; and b. a rotation source to rotate said inner and outer chambers about said central longitudinal axis.