Method for In-Vivo Binding of Chromatin Fragments

摘要

A process for substantially reducing levels of circulating chromatin fragments (CCFs) from a medium using binding agents such as antibodies or antibodies complexed with haemocompatible natural polymer substrates like as alginates, chitosan and pullulan to form complexed antibodysubstrate nano-particulates (CNP) to bind and/or inactivate CCFs is disclosed. The amount of antibody bound to the polymer varies from 30% to 100% of activated sites in the polymer. Elevated levels of CCFs can be substantially reduced following administration of tissue damaging agents that generate apoptotic chromatin fragments by the concomitant administration of CNPs or concomitant administration of H4 antibody alone. A method of treatment is disclosed wherein therapeutic dose of CNPs, or H4 antibody alone, are administered systematically, or orally, in a delivery system to curb pathological conditions that are associated with increased burden of circulating chromatin fragments.

主权项

1. A method of making a nanoparticle comprising a polymer having a selective affinity to liver cells of a mammal, and an antibody that binds or inactivates circulating chromatin fragments (CCFs), the method comprising:
forming an activated polymer, forming an activated antibody, forming complexed antibody substrate nanoparticulates (CNPs) by reacting the activated polymer and the activated antibody.