Screening Polynucleotide Libraries For Variants That Encode Functional Proteins

摘要

The present invention provides methods based on screening expressed polynucleotide libraries for soluble proteins.

主权项

1. A method of enriching a plurality of polynucleotides for polynucleotides likely to encode functional polypeptides and screening the enriched plurality, the method comprising:
(a) providing a plurality of polynucleotides encoding variants of a polypeptide, wherein at least some of the polynucleotides encode a polypeptide having at least one activity; (b) producing polypeptides from the polynucleotides; (c) determining whether the polypeptides are soluble; (d) selecting the polynucleotides that encode soluble polypeptides to form an enriched plurality of polynucleotides; and (e) screening the enriched plurality for polynucleotides encoding polypeptides having the activity.