A method of predicting relative fed batch production titer of a panel of clonally-derived producer cells

摘要

A computer implemented method of predicting relative fed batch production titer of a panel of clonal producer cells derived from a single parental host cell population comprises the steps of assaying a level of growth of each clone in the presence and absence of one or more individual chemical cell stressors, comparing the level of growth of each clone in the presence and absence of the or each chemical cell stressor to provide a normalised production titer response for each clone in the or each stressed microenvironment, and inputting a clone-specific production titer response profile comprising the normalised production titer response for each clone in the or each stressed microenvironments into a computational model. The computational model is generated from production titer response profiles obtained from a calibration set of clones with known fed batch production titer, and is configured to output the predicted relative fed batch production titer of the panel of clonal cells.