| 摘要 |
<p>There are provided, firefly luciferase with excellent thermostability and/or storage stability and a process for its production. Firefly luciferase having the amino acid sequence of firefly luciferase, wherein the amino acid corresponding to position 287 of Heike firefly luciferase has been replaced with alanine, or wherein the amino acid corresponding to position 392 has been replaced with isoleucine, and a gene for the firefly luciferase. By utilizing this gene, it is possible to efficiently produce firefly luciferase with increased stability. It is also possible to obtain firefly luciferase with further increased stability by combination with a mutation in which the amino acid at position 326 has been replaced with serine, and/or a mutation in which the amino acid at position 467 has been replaced with isoleucine.</p> |
