Method and device for detecting nucleic acid, and kit

摘要

The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.

主权项

1. A method for detecting a multi-stranded nucleic acid, comprising a step of bringing a chromogenic leuco dye into contact with a multi-stranded nucleic acid, wherein a color formed by interaction between the leuco dye and the multi-stranded nucleic acid is detected under visible light, and wherein the leuco dye is a compound represented by General Formula (I):wherein R1, R2 and R3 each independently represent a substituted or unsubstituted aryl group, and L represents —SO3R4, —NO3,—NO2, —CN, —X, —NHR5, —N(COR6)(COR7), —SR8, —SSR9, —OR10, —NHSNH2, —OH or —H, with R4 representing an alkali metal or a hydrogen atom; X representing a halogen atom; and R5, R6, R7, R8, R9 and Rl0 each independently representing an alkyl group, aryl group, acyl group, alkenyl group or alkynyl group, and
wherein a leaving group of the leuco dye is dissociated by interaction between the leuco dye and the multi-stranded nucleic acid, and a color that can be detected under visible light is then formed by dissociation of the leaving group.