Methods for affecting homology-directed DNA double stranded break repair

摘要

Disclosed herein are methods and composition for alteration of the presence, activity, or concentration of RNF138. Disclosed methods can be utilized to affect DNA double strand break repair. Methods and compositions can be utilized to alter RNF138 so as to prevent interaction between RNF138 and RAD51D. Specifically, methods can prevent RNF138-directed ubiquitylation of RAD51D, which is necessary for DNA double strand break repair.

主权项

1. A composition for affecting repair of DNA double strand breaks comprising RNF138 siRNA and further comprising a DNA damage reagent, wherein interaction between the Rnf138 si RNA and a proficient RNF138 cell reduces express of RNF138 and decreases repair of DNA double stranded breaks within the proficient RNF138 cell.