Monoclonal antibodies against glycoprotein of Ebola sudan boniface virus

摘要

We disclose Ebola Sudan Boniface virus GP Monoclonal antibodies, epitopes recognized by these monoclonal antibodies, and the sequences of the variable regions of some of these antibodies. Also provided are mixtures of antibodies of the present invention, as well as methods of using individual antibodies or mixtures thereof for the detection, prevention, and/or therapeutic treatment of Ebola Sudan Boniface virus infections in vitro and in vivo.

主权项

1. An isolated monoclonal antibody comprising a heavy chain variable region having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 8 and a light chain variable region having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 10,
wherein the monoclonal antibody binds Ebola Sudan Boniface virus (ESB) glycoprotein (GP); wherein the heavy chain variable region comprises amino acid residues Glu23, Val24, and Lys99, of the amino acid sequence of SEQ ID NO: 8; wherein the heavy chain variable region comprises:
a CDR1 comprising amino acid residues 48-55 of SEQ ID NO: 8;a CDR2 comprising amino acid residues 73-80 of SEQ ID NO: 8; anda CDR3 comprising amino acid residues 119-130 of SEQ ID NO: 8; and wherein the light chain variable region comprises:
a CDR1 comprising amino acid sequence of SEQ ID NO: 23;a CDR2 comprising amino acid sequence of SEQ ID NO: 24; anda CDR3 comprising amino acid sequence of SEQ ID NO: 25.