发明名称 |
Method for the determination of botulinum neurotoxin biological activity |
摘要 |
The present invention relates to means and methods for determining neurotoxin activity. Specifically, it relates to a polypeptide having caspase activity comprising a large subunit and a small subunit wherein the caspase further comprises a neurotoxin cleavage site which upon cleavage activates the caspase activity. Also encompassed are polynucleotides encoding the polypeptides as well as vectors or host cells comprising the polynucleotides. The present invention further relates to a method for determining neurotoxin activity in a sample based on the polypeptide of the invention as well as the use of the polypeptide for determining neurotoxin activity in a sample, in general. |
申请公布号 |
US9096886(B2) |
申请公布日期 |
2015.08.04 |
申请号 |
US201214004259 |
申请日期 |
2012.03.09 |
申请人 |
MERZ PHARMA GmbH & CO. KGaA |
发明人 |
Fink Klaus;Vey Martin |
分类号 |
A61K38/00;A61K38/48;C12Q1/37;G01N33/50;C12N9/64 |
主分类号 |
A61K38/00 |
代理机构 |
Hueschen and Sage |
代理人 |
Hueschen and Sage |
主权项 |
1. A polypeptide which exhibits caspase activity comprising a caspase large subunit and a caspase small subunit and a Clostridium botulinum or a Clostridium tetani neurotoxin cleavage site which upon cleavage activates the caspase activity. |
地址 |
Frankfurt am Main DE |