Recombinant nonpathogenic MDV vector providing multivalent immunity

摘要

The present invention relates to the field of veterinary vaccines, in particular to that of vector vaccines for poultry based on recombinant nonpathogenic Marek's disease virus (npMDV). The recombinant npMDV of the invention expresses stably and effectively two heterologous genes each originating from a different micro-organism: the fusion protein gene from Newcastle disease virus, and the viral protein 2 gene from infectious bursal disease virus. A vaccine based on this recombinant npMDV can be used to induce in poultry a protective immune response not only against Marek's disease, but also against Newcastle disease and Infectious bursal disease. The invention also relates to methods and uses involving the recombinant npMDV, expression cassette, infected host cells, and vaccines.

主权项

1. A recombinant nonpathogenic Marek's disease virus (npMDV) comprising a heterologous nucleic acid molecule, characterised in that the nucleic acid molecule comprises in 5′ to 3′ direction, and in this order:
a. a human cytomegalovirus immediate early 1 gene (hCMV-IE1) core promoter consisting of SEQ ID NO:1, or a homolog or variant of said hCMV-IE1 core promoter at least 95% identical to SEQ ID NO:1 and 271-451 nucleotides in size, b. a Newcastle disease virus (NDV) fusion (F) protein gene, c. a transcription terminator, d. a an adapted chicken beta-actin gene promoter consisting of SEQ ID NO:4, or a homolog or variant of said adapted promoter at least 95% identical to SEQ ID NO:4and 522-870 nucleotides in size, and e. a classic type infectious bursal disease virus (IBDV) viral protein 2 (VP2) gene.