发明名称 |
Method, system, and compositions for cell counting and analysis |
摘要 |
The present invention provides a low cost imaged-based system for detecting, measuring and/or counting labeled features of biological samples, particularly blood specimens. In one aspect, the invention includes a system for imaging multiple features of a specimen that includes one or more light sources capable of successively generating illumination beams each having a distinct wavelength band and a plurality of differentially excitable labels capable of labeling a specimen comprising multiple features, such that each different feature is labeled with a different differentially excitable label. |
申请公布号 |
US9097640(B2) |
申请公布日期 |
2015.08.04 |
申请号 |
US201213590114 |
申请日期 |
2012.08.20 |
申请人 |
Becton, Dickinson and Company |
发明人 |
Goldberg Edward Michael;Brooker Jeffrey |
分类号 |
G01N1/10;G01N21/03;B01L3/00;G01N21/64;G01N33/50;G01N15/14;G01N33/49;G01N15/10 |
主分类号 |
G01N1/10 |
代理机构 |
Bozicevic, Fields & Francis LLP |
代理人 |
Field Bret E.;Bozicevic, Fields & Francis LLP |
主权项 |
1. An apparatus for analyzing in a blood specimen non-red cells labeled with a plurality differentially excitable labels, the apparatus comprising:
a sample chamber having a top wall and a bottom wall capable of containing a blood specimen, wherein the distance between the top wall and the bottom wall is configured to preclude the formation of a light-obstructing layer of red blood cells; a blood specimen present in the sample chamber, wherein the blood specimen is labelled with a plurality of differentially excitable labels positioned in the sample chamber, such that each different non-red cell component of the blood specimen is labeled with a different differentially excitable label that emits an optical signal within the same wavelength band; multiple light sources each capable of illuminating the blood specimen with an illumination beam having a distinct wavelength band; a controller coupled to the multiple light sources for successively directing the illumination beam of each light source onto the specimen; an optical system capable of collecting such emitted optical signals and forming successive images corresponding thereto on a light-responsive surface to form successive sets of image data; and enumerating the non-red cells in the blood specimen by analyzing the successive sets of image data. |
地址 |
Franklin Lakes NJ US |