Antibodies for determining the prothrombin fragment F2/F1+2 in a homogeneous immunoassay

摘要

The present invention is in the area of coagulation analysis and relates to antibodies which bind specifically to an immune complex of prothrombin fragment F2/F1+2 and an F2/F1+2 neoepitope-specific antibody fragment, and to the preparation and the use thereof in methods for determining F2/F1+2.

主权项

1. An isolated immune complex comprising an antibody fragment and a peptide, wherein:
the peptide includes at least a complete prothrombin fragment F2; the carboxy terminus of the complete prothrombin fragment F2 of the peptide is bound to the antibody fragment; the antibody fragment has specificity for a carboxy-terminal neoepitope of the prothrombin fragment F2 and/or fragment F1+2; and wherein the antibody fragment is a fragment of a monoclonal antibody made by hybridoma cell line DSM ACC 2911.