| 主权项 |
1. A process for the biocatalytic production of a β-amino acid precursor, comprising:
reacting at least one substrate of the general formula (I) wherein R1 and R2 independently from each other are selected from:
hydrogen;a linear or branched, optionally substituted, lower alkyl group;a linear or branched, optionally substituted, lower alkenyl group;an optionally substituted cyclic alkyl group;a mono- or polycyclic, optionally substituted aryl group;a mono- or polycyclic, optionally substituted heteroaryl group;a linear or branched, optionally substituted alkoxy group;an amino group;a linear or branched, optionally substituted alkylamino group;a linear or branched, optionally substituted alkylthio group;a linear or branched, optionally substituted acyl group,a carboxyl group oran aldehyde group;said substrate being present in stereoisomerically pure form or as a mixture of stereoisomers, wherein the stereoisomerically pure form or the mixture of stereoisomers may optionally be a salt, in the presence of at least one enzyme, catalyzing the hydrolytic cleavage of a hydantoin and/or dihydropyrimidin ring, so that a β-amino acid precursor of the general formula (II) wherein R1 and R2 are identified as above, is produced; said process being characterized in that the at least one enzyme which is applied in said process is a) an enzyme having hydantoinase and /or dihyropyrimidinase activity which is obtained from Vigna angularis and comprising at least one of the amino acid sequences of SEQ ID NO: 5 and SEQ ID NO: 7; or b) an enzyme having 1 to 20 amino acid additions, substitutions, deletions and/or inversions relative to the enzyme of a), wherein said enzyme comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 5 or SEQ ID NO 7, and has hydantoinase and/or dihydropyrimidinase activity. |
