| 摘要 |
<p>A method for the detection of small molecules in a sample comprises a) coupling a nanoparticle (NP) or microparticle (MP) to an aptamer specific for the small molecule to be detected to form a NP-aptamer conjugate; b) contacting the NP-aptamer conjugate with the sample; and c) detecting a reduction in the size, a change in surface potential, or a change in mobility of the NP-aptamer conjugate. The reduction or change is indicative of the presence of the small molecule. A biosensor comprising (a) a nanoparticle or microparticle and (b) an aptamer useful in the detection of a small molecule in a sample is disclosed. The nanoparticle or microparticle is coupled to the aptamer to provide an NP-aptamer conjugate, and where there is a reduction in the size, a change in surface potential, and/or a change in mobility of the NP-aptamer conjugate when the NP-aptamer conjugate is contacted with a small molecule in a sample. Also disclosed is an aptamer having a sequence as defined in the specification.</p> |
