| 摘要 |
The present invention provides a binary process for simultaneous detection of Salmonella sp., Shigella sp., Proteus sp. and E. coli in a sample. In one aspect, the process includes steps of carrying out dot ELISA using monoclonal antibodies generated and characterized against outer membrane protein of Shigella flexneri and whole cell lysate of Salmonella typhimurium for detecting the presence of Salmonella sp., Shigella sp., Proteus sp. and E. coli in the sample, and conducting a set of biochemical tests for confirming presence of the Salmonella sp., Shigella sp., Proteus sp. and E. coli in the sample. The present invention also provides for the monoclonal antibodies generated and characterized against outer membrane protein of Shigella flexneri and whole cell lysate of Salmonella typhimurium. In another aspect, the present invention provides a kit for simultaneous detection of Salmonella sp., Shigella sp., Proteus sp. and E. coli in a sample involving an antigen-antibody reaction. Figure 3 |
