| 摘要 |
<p>Disclosed is a method for isolating osteopontin from a milk-derived feed, the method comprising the steps of: a) providing a milk-derived feed comprising osteopontin, said milk derived feed having a pH in the range of pH 3.6-6.5 at 25 degrees C and a specific conductance in the range of 4-10 mS/cm at 25 degrees C, and wherein the milk-derived feed comprises a total amount of protein in the range of 50-250 g/L milk-derived feed, b) subjecting said milk-derived feed to anion exchange chromatography, which includes contacting the milk-derived feed with an anion exchange medium, c) optionally, washing the anion exchange medium, and d) recovering the protein bound to the anion exchange medium, thereby obtaining a composition comprising isolated osteopontin. Reference has been directed, in pursuance of Section 16(1) of the Patents Act 1953, to NZ 614872.</p> |
