Ratio based biomarkers and methods of use thereof

摘要

A method of determining cancer prognosis for a subject with a cancer is disclosed. At least two cancer associated proteins in a sample from the subject are identified at least two cancer associated proteins comprise p-MAPK and EGFR but not p-mTOR or p-AKT. The at least two cancer associated proteins in the sample are quantified. The at least two cancer associated proteins in the sample are normalised to obtain a normalized value for each cancer associated protein in the sample. The normalized value of the first cancer associated protein is compared with the normalized value of the second cancer associated protein to obtain a biomarker indicator that comprises a ratio of the normalized value of the first cancer associated protein to the normalized value of the second cancer associated protein; and the biomarker indicator is correlated with prognosis of the subject with cancer.