发明名称 |
METHODS AND SYSTEMS FOR IDENTIFYING CRISPR/CAS OFF-TARGET SITES |
摘要 |
Methods and systems for searching genomes for potential CRISPR off-target sites are provided. In preferred embodiments, the methods include identifying possible on- and off-target cleavage sites and /or ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the gRNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome. These methods allow for the selection of better target sites and/or experimental confirmation of off-target sites and are an improvement over partial search mechanisms that fail to locate every possible target site. |
申请公布号 |
WO2015113063(A1) |
申请公布日期 |
2015.07.30 |
申请号 |
WO2015US13134 |
申请日期 |
2015.01.27 |
申请人 |
GEORGIA TECH RESEARCH CORPORATION |
发明人 |
CRADICK, THOMAS JAMES;BAO, GANG;QIU, PENG |
分类号 |
G06F19/22 |
主分类号 |
G06F19/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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