PREPARING METHOD OF SAMPLE, AND IMMUNOASSAY METHOD OF VITAMIN

摘要

PROBLEM TO BE SOLVED: To provide a method for accurately measuring the concentration of a vitamin in a blood sample subjected to alkali treatment.SOLUTION: By mixing a surfactant (preferably a nonionic surfactant such as Nonidet P-40) with a blood sample subjected to alkali treatment, a blood sample mixture for measuring a vitamin (preferably a vitamin D metabolite, such as 25-hydroxyvitamin Dand 25-hydroxyvitamin D, vitamin Bor folic acid) is prepared. The vitamin is measured with an immunoassay as it is, without separation or extraction from the blood sample mixture for measurement.