发明名称 Methods of Screening for Susceptibility to Virus Infection
摘要 The invention is directed to methods for screening the resistance of CD4+ cells to viral infection, in particular human immunodeficiency virus (HIV) infection.
申请公布号 US2015211063(A1) 申请公布日期 2015.07.30
申请号 US201314427595 申请日期 2013.09.11
申请人 THE HENRY M. JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE, INC. 发明人 Hu Haitao;Kim Silvia R.
分类号 C12Q1/68;C12Q1/70;G01N33/50 主分类号 C12Q1/68
代理机构 代理人
主权项 1. A method for screening the resistance of CD4+ cells to viral infection, the method comprising a) labeling isolated CD4+ cells with carboxyfluorescein diacetate, succinimidyl ester (CFSE), b) contacting the labeled CD4+ cells with at least one composition to stimulate the CD4+ cells, c) contacting the stimulated, labeled CD4+ cells with a subject virus, and d) determining the infectivity of the subject virus in the stimulated, labeled CD4+ cells, wherein a lower viral infectivity in the stimulated, labeled CD4+ cells compared to control cells indicates that the stimulated, labeled CD4+ cells are more resistant to infection of the virus than control cells.
地址 Bethesda MD US