| 摘要 |
The invention relates to a method for the simultaneous detection of at least four nucleic acids labeled with a covalently attached dye in a sample comprising the step of: detecting the fluorescence emission of said fluorescent dyes upon excitation, wherein the at least four dyes attached to said nucleic acids are selected from the groups of: i. 6-FAM or 5-FAM or a blend thereof covalently attached to a first nucleic acid, ii. DY-530, HEX or ATTO 532 or a blend thereof covalently attached to a second nucleic acid, iii. ATTO550 or DY-555 or a blend thereof covalently attached to a third nucleic acid, iv. ATT0565, DY510-XL or ROX or a blend thereof covalently attached to a fourth nucleic acid, v. DY632 or DY520-XL or a blend thereof covalently attached to a fifth nucleic acid and, vi. Chromeo 494 attached to a sixth nucleic acid, wherein Chromeo 494 must be one of the four selected. |
