发明名称 Methods For Enhancing The Degradation Or Conversion Of Cellulosic Material
摘要 The present invention relates to processes for degrading a cellulosic material and for producing substances from the cellulosic material using recombinant glycoside hydrolase of family 61 (GH61) from Trichoderma.
申请公布号 US2015210991(A1) 申请公布日期 2015.07.30
申请号 US201314429241 申请日期 2013.09.19
申请人 NOVOZYMES, INC. ;NOVOZYMES A/S 发明人 Schnorr Kirk;Shaghasi Tarana;McBrayer Brett
分类号 C12N9/42;C12P19/02;C12P19/14 主分类号 C12N9/42
代理机构 代理人
主权项 1. A process for degrading a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a GH61 polypeptide having cellulolytic enhancing activity selected from the group consisting of: (a) a GH61 polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, or at least 93% sequence identity to the mature polypeptide of SEQ ID NO: 6; (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, (ii) the cDNA sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or (iii) the full-length complement of (i) or (ii); (c) a GH61 polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 7, or SEQ ID NO: 8, or the cDNA sequence of SEQ ID NO: 1 or SEQ ID NO: 3, or at least 93% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5 or SEQ ID NO: 9; (d) a variant of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 6 comprising a substitution, deletion, and/or insertion at one or more positions; and (e) a fragment of the GH61 polypeptide of (a), (b), (c), or (d) that has cellulolytic enhancing activity.
地址 Davis CA US