| 发明名称 | Biomarker DACT1 for gastric cancer | ||
| 摘要 | The present invention provides a method for diagnosing and determining prognosis of gastric cancer in a subject by detecting suppressed expression of the DACT1 gene, which in some cases is due to elevated methylation level in the genomic sequence of this gene. A kit and device useful for such a method are also provided. In addition, the present invention provides a method for treating gastric cancer by increasing DACT1 gene expression or activity. | ||
| 申请公布号 | US9090944(B2) | 申请公布日期 | 2015.07.28 |
| 申请号 | US201113328785 | 申请日期 | 2011.12.16 |
| 申请人 | The Chinese University of Hong Kong | 发明人 | Yu Jun;Sung Joseph Jao Yiu;Wang Shiyan |
| 分类号 | C12Q1/68;G01N33/574 | 主分类号 | C12Q1/68 |
| 代理机构 | Kilpatrick Townsend & Stockton LLP | 代理人 | Kilpatrick Townsend & Stockton LLP |
| 主权项 | 1. A method for detecting gastric cancer in a subject, comprising the steps of: (a) treating a gastric sample taken from the subject with a bisulfite; (b) performing a polymerase chain reaction using a primer consisting of the polynucleotide sequence of SEQ ID NO:9, 10, 11, 12, 13, or 14 and determining methylation status of each CpG in a CpG-containing genomic sequence, wherein the CpG-containing genomic sequence is the -465 to -406 -465 to -406 region from human DACT1 transcription start site or a fragment thereof that comprises at least one CpG; and (c) diagnosing the subject as having gastric cancer when at least one CpG in the CpG-containing genomic sequence is at least 11% methylated. | ||
| 地址 | Shatin, N.T. HK | ||