| 摘要 |
Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate). |
| 主权项 |
1. A recombinant organism genetically engineered to convert glutarate semialdehyde into a 5-hydroxyvalerate monomer, polymer or copolymer thereof,
wherein the recombinant organism expresses at least two or more heterologous genes encoding two or more enzymes selected from the group consisting of: lysine 2-monooxygenase, EC 1.13.12.2; 5-aminopentanamidase (δ-aminovaleramidase), EC 3.5.1.30; 5-aminovalerate transaminase, EC 2.6.1.48; lysine decarboxylase, EC 4.1.1.18; glutarate semialdehyde reductase, EC 1.1.1.61; 4-hydroxybutyrate dehydrogenase, EC 1.1.1.61; CoA-transferase, EC 2.8.3.14 and EC 2.8.3.n; Acyl-CoA synthetase, EC 6.2.1.3; PHA synthase, EC 2.3.1.n; β-ketoacyl-CoA thiolase, EC 2.3.1.9; acetoacetyl-CoA reductase, EC 1.1.1.36; propionaldehyde dehydrogenase, EC 1.2.1.3; alcohol dehydrogenase, EC 1.1.1.1; and 1,3-propanediol dehydrogenase EC 1.1.1.202; wherein the recombinant organism produces more 5-aminopentanoate than an unmodified organism; and wherein the 5-aminopentanoate is converted into the 5-hydroxyvalerate monomer, polymer or copolymer thereof by the recombinant organism wherein the 5-hydroxyvalerate monomer, polymer or copolymer thereof is isolatable. |
