Devices and methods for biological sample preparation

摘要

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

主权项

1. A device comprising:
a top structure, a bottom structure, and a connecting structure, wherein the connecting structure connects the top structure to the bottom structure, and wherein
I) the top structure comprises
a) a first plurality of containers, each container of the first plurality of containers having:
i) a top opening, wherein the top opening has a sealing element which can slide inside the container while substantially maintaining a seal;ii) a central part; andiii) a bottom opening;b) at least two ports in at least one container of the first plurality of containers, the at least two ports comprising
i) a first port configured for air venting;ii) a second port configured for fluid insertion; andiii) a sealing cap for each port;II) the bottom structure comprises
a) a second plurality of containers, each container of the second plurality of containers having
i) a top opening;ii) a central part; andiii) a bottom opening, wherein the bottom opening has a sealing element; andIII) the connecting structure comprises
a) at least one fluidics channel;b) a supporting element which can slide, rotate or move within a plane, thereby establishing a fluidics connection, through the at least one fluidics channel, between the bottom opening of at least one container of the first plurality of containers and the top opening of at least one container of the second plurality of containers, while substantially sealing other bottom openings of the first plurality of containers and other top openings of the second plurality of containers.