Olfactory epithelial-derived stem cells and methods of use therefor

摘要

The presently disclosed subject matter provides recombinant dopaminergic neurons and/or recombinant progenitors thereof that include one or more transgenes encoding a nurr-1 polypeptide, a pitx3 polypeptide, an Imxi a polypeptide, a biologically active fragment thereof, a biologically active derivative thereof, and/or any combination thereof. Also provided are methods for producing a recombinant dopaminergic neuron or recombinant progenitor thereof, methods for producing lineage primed cells, methods for ameliorating at least one symptom associated with a neurological disorder, methods for transplantation, methods for inducing growth, repair, ancllor regeneration of a neuron, for delivering a cytokine or a growth factor to the central nervous system, methods for providing a dopaminergic neuron function, and cell cultures that include recombinant olfactory epithelial-derived stem cells and/or a differentiated derivatives thereof.

主权项

1. A method for producing a recombinant dopaminergic neuron, the method comprising:
(a) providing adult human olfactory epithelial-derived stem cells; (b) introducing, into the adult human olfactory epithelial-derived stem cells, one or more transgenes encoding a nurr-1 polypeptide, a pitx3 polypeptide and a Imx1a polypeptide, to produce transgenic adult human olfactory epithelial-derived stem cells; and (c) culturing the transgenic adult human olfactory epithelial-derived stem cells to produce recombinant dopaminergic neurons, wherein the recombinant dopaminergic neurons produce increased levels of intracellular dopamine relative to the levels of intracellular dopamine produced by adult human olfactory epithelial-derived stem cells expressing the nurr-1 polypeptide, the pitx3 polypeptide or the Imx1a polypeptide.