Oligonucleotide duplexes comprising DNA-like and RNA-like nucleotides and uses thereof

摘要

Novel oligonucleotide pairs which can form a duplex comprising one or more DNA-like nucleotides (e.g., 2′-sub-stituted arabinonucleotides (ANA)); in combination with one or more RNA-like nucleotides (e.g., 2′-substituted ribonucleotides (RNA) and/or locked nucleic acid nucleotides (LNA)), are disclosed. The use of such oligonucleotide duplexes, such as for silencing the expression of a nucleic acid or gene of interest using small interfering RNA (siRNA) technologies, is also disclosed.

主权项

1. A hybridizable oligonucleotide pair for forming a duplex comprising a sense strand having a 5′ end and an antisense strand complementary to the sense strand,
the sense strand comprising a region 19 to 23 residues in length complementary to the antisense strand and consisting of: a plurality of 2′F-ANA and 2′F-RNA nucleotides, wherein at least a portion of the 2′F-ANA and 2′F-RNA nucleotides are present in a first altimer and a second, smaller altimer where the first altimer is located closer to the 5′ end of the sense strand than the second altimer; and the antisense strand comprising a region 19 to 23 residues in length complementary to the sense strand and consisting of: a plurality of 2′F-RNA nucleotides or RNA nucleotides.