Microscope and method for the microscopic detection of light of a sample

摘要

A microscope or use of a microscope with at least one illumination beam that is phase-modulated along its cross section partially with a modulation frequency, in which an advantageously local excitation of a transition, advantageously a fluorescence excitation in a sample, is performed by means of a pump beam of a first wavelength and the transition is induced and detected advantageously by means of a second beam, advantageously of a second wavelength, or a local heating is generated that is read out from the detection signals by means of a detection unit, advantageously an infrared camera.

主权项

1. A microscope comprising at least one light source providing an illumination beam that is phase-modulated along its cross section partially with a temporal modulation frequency for excitation of a sample, an electro-optical modulator EOM located only partially in said illumination beam, said at least one light source being a first light source providing a first beam of a first wavelength for performing local excitation of a transition of said sample, a second light source providing a second beam for inducing said transition, and a detection unit for reading out and detecting said transition.