Treatment of sirtuin (SIRT) related diseases by inhibition of natural antisense transcript to a sirtuin (SIRT)

摘要

The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of a Sirtuin (SIRT), in particular, by targeting natural antisense polynucleotides of a Sirtuin (SIRT). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Sirtuins (SIRT)s.

主权项

1. A method of upregulating function of and/or the expression of a Sirtuin (SIRT) gene/ polynucleotide selected from the group consisting of SIRT 3, 4, 5 and 7 in patient cells or tissues in vivo or in vitro comprising: contacting said cells or tissues with at least one antisense oligonucleotide of 10-30 nucleotides in length that specifically targets a complementary region of a natural antisense oligonucleotide of the Sirtuin (SIRT) polynucleotide wherein said natural antisense oligonucleotide is selected from the group consisting of polynucleotides 1 to 726 of SEQ ID NO: 17, 1 to 320 of SEQ ID NO: 18, 1 to 616 of SEQ ID NO: 19, 1 to 492 of SEQ ID NO: 20, 1 to 705 of SEQ ID NO: 23 or 1 to 2714 of SEQ ID NO: 141 or 1 to 1757 of SEQ ID NO: 142 or 1 to 3647 of SEQ ID NO: 143; and wherein said oligonucleotide is specifically hybridizable thereto thereby upregulating a function of and/or the expression of the Sirtuin (SIRT) polynucleotide in patient cells or tissues in vivo or in vitro.