Method for screening of therapeutic agent for hyperlipemia

摘要

Disclosed are a highly safe treatment method for hyperlipidemia and a therapeutic agent for hyperlipidemia. Specifically, the invention provides a novel method for screening an agent for treating hyperlipidemia, more specifically, a method for screening a substance that can inhibit the production or function of gangliosides, particularly GM3, or inhibit the activity or expression of GM3 synthase to reduce a blood lipid level. A pharmaceutical composition, which can specifically inhibit the production of gangliosides, particularly GM3, thereby effective for hyperlipidemia treatment, and others are also provided.

主权项

1. A method for screening a GM3 synthase specific inhibitor having a blood lipid level lowering activity that specifically inhibits GM3 synthase expression, which comprises:
contacting a test substance with a cell selected from the group consisting of an adipocyte and a hepatocyte, wherein the cell expresses GM3 synthase; selecting a test substance that specifically decreases the expression level of GM3 synthase as compared to a control; administering the selected test substance to a non-human apoE-deficient animal; and determining a Chylomicron (CM) level, a Very Low Density Lipoprotein (VLDL) level or a Low Density Lipoprotein (LDL) level in blood in the non-human apoE-deficient animal and selecting a test substance having a blood lipid level lowering activity that specifically inhibits GM3 synthase expression and reduces the CM, VLDL or LCL level in blood by 10% or more as compared to a control.