Method of preventing or treating influenza A viral infection using cloned DI influenza A viral particles

摘要

Cloned, i.e. defined, defective interfering (DI) influenza A virus is produced in embryonated hens eggs using a method which generates large quantities of DI virus material. Co-administration of cloned DI influenza A virus with a lethal dose of virulent influenza A virus conferred protection in mice compared to a control of inactivated cloned DI influenza A virus. Control mice which received only cloned DI influenza A virus and no lethal challenge of virulent influenza A virus were not protected three weeks later on lethal challenge with infective virus. Cloned DI influenza A virus of one subtype is found to act in vivo as an effective antiviral against the same or any other sub-type of influenza A virus. The antiviral effect has been found to have both a therapeutic and a prophylactic application against influenza A infection in humans, mammals and birds.

主权项

1. A method for preventing or treating influenza A in a subject comprising administering an effective amount of a cloned defective interfering (DI) influenza A virus particle, wherein the cloned DI influenza A virus particle comprises an RNA segment 1 of the cloned DI influenza A virus;
further wherein the RNA segment 1 comprises a nucleic acid sequence consisting of (a) a sequence selected from SEQ ID NO: 1 or SEQ ID NO: 2; or (b) a nucleic acid sequence having at least 90% sequence identity with SEQ ID NO: 1.