Identifcation of CD8⁺ T cells that are CD161^hiand/or IL18Rα^hiand have rapid drug efflux capacity

摘要

This invention provides, among other things, methods for the identification and isolation of viable putative long-lived antigen-specific memory CD8+ T cell subsets (CMhi and EMhi) with high surface expression of CD161 and/or IL-18Rα and the capacity to rapidly efflux the fluorescent dye Rh123.

主权项

1. A composition comprising: a) a physiological acceptable carrier and b) an isolated population of long-lived memory CD8+ CD161hi IL-18Rαhi T cells, wherein:
the long-lived memory CD8+ CD 161hi IL-18Rαhi T cells make up at least 30% of the total CD8+ T cells in the composition; the long-lived memory CD8+ CD161hi IL-18Rαhi T cells comprise CD95hi memory cells, the CD95hi memory cells being capable of proliferating in response to IL-7 or IL-15 and comprising a population of cells comprising an engineered immunoreceptor; and where the isolated population of long-lived memory CD8+ CD161hi IL-18Rαhi T cells comprises high CD28 surface expression and high MDR-1 mRNA levels, as compared to a CD8+ T cell population with low surface expression of IL-18Rα.