METHOD FOR PREPARING A BONE PROTEIN PREPARATION AND A BONE PROTEIN PREPARATION

摘要

The present invention provides a method for preparing a bone protein preparation which contains for example growth factors. The present invention also provides a bone protein preparation obtained by the method and paste, putty, pellet, disc, block, granule, osteogenic device or pharmaceutical composition containing said bone protein preparation.

主权项

1. A method of making a bone protein preparation comprising:
a) demineralizing a bone and extracting the bone matrix with guanidine hydrochloride solvent to obtain a bone protein extract, b) filtering the extract with a microfilter with cut-off size in the range of 0.1-10 μm (nominal micron rating) sufficient for removing big particles and non-proteinous material but enabling proteins to pass, c) filtering the flow-through with a cassette ultrafilter having the cut-off size about 5-10 kDa to recover the bone protein preparation, and d) incorporating the bone preparation in a polyethylene glycol/glycerol (PEG-GLY) matrix.