BEVEL-AXIAL AUTO-FOCUSING MICROSCOPIC SYSTEM AND METHOD THEREOF

摘要

An embodiment disclosed bevel-axial auto-focusing method for a microscopic system, including: capturing full-frame bevel-axial input image; using image analysis to read image information and transmitting grayscale information to a statistic analysis module; the statistic module extracting image statistic characteristics and performing curve fitting with probability function; and estimating the optimal focus point based on the post-fitting characteristic parameters. A preferred embodiment combines image with Gaussian curve fitting and Kalman filter, and analyzes the in-focus position based on image quality so as to directly determine the optimal rectification value of the in-focus position on the object surface. The method can improve the accuracy and speed of auto-focusing.

主权项

1. A bevel-axial microscopic auto-focusing system, comprising:
an optical microscopic system, a charge-coupled device (CCD), an image-capturing card, a controller and a motor driver; wherein the optical microscopic system further including a light source, a first lens, a spectroscope, a second lens and a microscope objective, disposed in sequence from top down for observing an object; the microscope objective being for magnifying an image of the object; the light source emitting light, the emitted light travelling through the first lens, the spectroscope, the second lens and the microscope objective to reach and be reflected by the object; the reflected light travelling through the microscope objective, the second lens and the spectroscope; the reflected light being rotated 90° by the spectroscope and travelling to the CCD disposed laterally to a side of the spectroscope; the CCD being disposed in a bevel-axial manner of forming a tilt angle θ between the surface of the CCD and a vertical axis; the controller using the image-capturing card to capture a microscopic image of the object through the bevel-axial CCD and controlling the motor driver to drive the microscope objective to provide focusing along the vertical axis to achieve auto-focusing.