发明名称 |
METHOD FOR MANUFACTURING CILIARY MARGIN STEM CELLS |
摘要 |
The present invention provides a method for manufacturing ciliary margin stem cells differentiated from pluripotent stem cells, the method comprising step (1) and/or step (2) below: (1) a step for obtaining retinospheres by suspension culturing of cells obtained from cell aggregates comprising ciliary margin-like structures differentiated from pluripotent stem cells; and (2) a step for isolating stage specific embryonic antigen-1-positive cells from cells obtained from cell aggregates comprising ciliary margin-like structures differentiated from pluripotent stem cells. The present invention makes it possible to manufacture efficiently and with high purity ciliary margin stem cells with the ability to differentiate to retinal cells comprising retinal layer-specific neurons and the ability to self-replicate. |
申请公布号 |
WO2015107738(A1) |
申请公布日期 |
2015.07.23 |
申请号 |
WO2014JP77603 |
申请日期 |
2014.10.16 |
申请人 |
SUMITOMO CHEMICAL COMPANY, LIMITED;RIKEN |
发明人 |
KUWAHARA, ATSUSHI;SASAI, YOSHIKI (笹井 芳樹) |
分类号 |
C12N5/071;A61K35/44;A61L27/00;A61P27/02;A61P43/00;C12N5/074;C12N5/10;C12Q1/02 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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