PEPTIDES, DEVICES, AND METHODS FOR THE DETECTION OF ANAPLASMA ANTIBODIES

摘要

The invention provides populations of isolated peptides useful for the detection of antibodies that bind to Anaplasma antigens. The peptide populations comprise peptides derived from immunogenic fragments of the Anaplasma Outer Membrane Protein proteins. The invention also provides devices, methods, and kits comprising the populations of isolated peptides useful for the detection of antibodies that bind to Anaplasma antigens and the diagnosis of anaplasmosis. Methods of identifying the particular Anaplasma species infecting a subject using the peptide populations of the invention are also disclosed.

主权项

1. A composition comprising a population of isolated peptides, said population comprising three or more different peptides, wherein each peptide in the population comprises a sequence of:
(i) Abaxis ID 2 (SEQ ID NO: 1) or a fragment thereof, wherein X9 is an amino acid selected from the group consisting of I, P or H, X17 is an amino acid selected from the group consisting of I, W, or Y, X21 is an amino acid selected from the group consisting of R, D, or N, X28 is an amino acid selected from the group consisting of E or N, and X31 is an amino acid selected from the group consisting of L or V; (ii) Abaxis ID 3 (SEQ ID NO: 2) or a fragment thereof, wherein X3 is an amino acid selected from the group consisting of L, V or A, X7 is an amino acid selected from the group consisting of K, N or Q, X11 is an amino acid selected from the group consisting of R, D, or N, and X15 is an amino acid selected from the group consisting of E, N or Q; (iii) APL-ID1 (SEQ ID NO: 3) or a fragment thereof, wherein X5 is an amino acid selected from the group consisting of V or A, X7 is an amino acid selected from the group consisting of G, I or H, X11 is an amino acid selected from the group consisting of E, N, or Q, X18 is an amino acid selected from the group consisting of D or N, X21 is an amino acid selected from the group consisting of R, D, or N, X25 is an amino acid selected from the group consisting of Q, D, or E, X28 is an amino acid selected from the group consisting of E or N, X31 is an amino acid selected from the group consisting of L or V, X45 is an amino acid selected from the group consisting of K or Q, X48 is an amino acid selected from the group consisting of F or V, X51 is an amino acid selected from the group consisting of D or N, X54 is an amino acid selected from the group consisting of E or Q, X57 is an amino acid selected from the group consisting of S or Q, X60 is an amino acid selected from the group consisting of F or W, X63 is an amino acid selected from the group consisting of I or V, and X66 is an amino acid selected from the group consisting of Q or D; (iv) APL-ID2 (SEQ ID NO: 4) or a fragment thereof, wherein X6 is an amino acid selected from the group consisting of K or Q, X9 is an amino acid selected from the group consisting of F or V, X12 is an amino acid selected from the group consisting of D or N, X15 is an amino acid selected from the group consisting of E or Q, X18 is an amino acid selected from the group consisting of S or Q, X21 is an amino acid selected from the group consisting of F or W, X24 is an amino acid selected from the group consisting of I or V, and X27 is an amino acid selected from the group consisting of Q or D; (v) APL-ID3 (SEQ ID NO: 5) or a fragment thereof, wherein X2 is an amino acid selected from the group consisting of I or V, X10 is an amino acid selected from the group consisting of S or Y, and X23 is an amino acid selected from the group consisting of E or N; (vi) APL-ID5.1 (SEQ ID NO: 6) or a fragment thereof, (vii) APL-ID6 (SEQ ID NO: 7) or a fragment thereof, wherein X5 is an amino acid selected from the group consisting of S or Q, X9 is an amino acid selected from the group consisting of F or Y, X13 is an amino acid selected from the group consisting of R or H, X16 is an amino acid selected from the group consisting of W or Y, X18 is an amino acid selected from the group consisting of S or Q, X22 is an amino acid selected from the group consisting of K or H, and X27 is an amino acid selected from the group consisting of N or D; (viii) APL-ID7 (SEQ ID NO: 8) or a fragment thereof, wherein X10 is an amino acid selected from the group consisting of V, L or I, and X11 is an amino acid selected from the group consisting of A or L; or (ix) APID 2-1 (SEQ ID NO: 9) or a fragment thereof, wherein X5 is an amino acid selected from the group consisting of V or A, X7 is an amino acid selected from the group consisting of G, I or H, X11 is an amino acid selected from the group consisting of E, N, or Q, X21 is an amino acid selected from the group consisting of R, D, or N, X25 is an amino acid selected from the group consisting of Q, D, or E, X28 is an amino acid selected from the group consisting of E or N, and X31 is an amino acid selected from the group consisting of L or V.