Screening Methods for Transfusion Related Acute Lung Injury (TRALI)

摘要

The invention relates to the discovery that HNA-3a and HNA-3b are antigens within a polypeptide sequence that is highly similar to the CTL2 amino acid sequence. This invention provides methods and kits for screening for HNA-3a and HNA-3b specific antibodies, HNA-3a and HNA-3b polypeptides and HNA-3a and HNA-3b nucleic acids in a sample of a biological tissue intended for transplantation

主权项

1. A method of determining whether a donor tissue intended for transplant or transfusion will induce transfusion related acute lung injury (TRALI) or graft versus host disease (GVHD) in a human recipient wherein the human recipient expresses the HNA-3a antigen comprising
a) obtaining a sample of the tissue intended for transplant or transfusion in the human subject, b) contacting the sample with a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof to form a complex with HNA-3a specific antibodies in the sample, and c) detecting the complex, wherein the presence of the complex indicates that the donor tissue is likely to induce TRALI or GVHD in a human recipient that expresses the HNA-3a antigen.