CELL CULTURE OF CORNEAL ENDOTHELIAL CELLS

摘要

The invention relates to a dual-media approach for culturing isolated corneal endothelial cells. Isolated corneal endothelial cells are first contacted with a proliferative medium to propagate and/or expand the endothelial cells followed by a maintenance medium to preserve the morphology and/or characteristics of the corneal endothelial cells. The invention includes the proliferative medium and the maintenance medium and also a combination of the two medium.

主权项

1. A method for culturing isolated corneal endothelial cells comprising the steps of:
(i) contacting isolated corneal endothelial cells with a proliferative medium to propagate and/or expand corneal endothelial cells; and (ii) contacting the propagated and/or expanded corneal endothelial cells with a maintenance medium to substantially preserve the morphology and characteristics of the corneal endothelial cells.