METHOD FOR PRODUCING ANTIBODY MOLECULES HAVING INTER-SPECIES, INTRA-TARGET CROSS-REACTIVITY

摘要

A method is disclosed for producing an antibody molecule having inter-species, intra-target cross-reactivity. The method comprises the steps of creating a first immune library for a target antigen from a first species, for example man; combining variable regions from the first immune library in a chain shuffling protocol, to produce a second immune library; and screening the second immune library for affinity to both the target antigen and a corresponding antigen from a second species. The second species is different from the first species. By using a suitable test animal as the second species, the method paves the way to convenient testing of the antibody molecule.

主权项

1. A method for producing an antibody molecule having inter-species, intra-target cross-reactivity, said method comprising the steps of:
a. creating a first immune library specific for a target antigen from a first species; b. combining variable regions from the first immune library in a chain shuffling protocol to produce a second immune library; c. identifying in the second immune library antibody molecules being reactive to the target antigen and to a corresponding antigen from a second species;wherein the second species is different from the first species.