Autologous Mammalian Models Derived from Induced Pluripotent Stem Cells and Related Methods

摘要

Disclosed is an autologous non-human mammalian model system derived from induced pluripotent stem (iPS) cells. Also disclosed are methods of differentiating non-human primate iPS cells, which can result in populations of cells enriched for SOX2+ or PDX1+ foregut-like cells, for CDX2+ hindgut-like cells, for CD34+ hematopoietic progenitor-like cells, or epithelial-like cells. Also disclosed is a non-human primate containing an autologous cell type of interest, which is differentiated in vitro from an induced pluripotent stem cell reprogrammed from a primary somatic cell. Methods of monitoring exogenously introduced cells within a non-human mammal are also disclosed.

主权项

1. An autologous non-human mammalian model system, comprising:
(i) introducing into a non-human mammal an autologous cell type of interest, wherein the cell type of interest is differentiated from an induced pluripotent stem cell reprogrammed from a primary somatic cell obtained from the non-human mammal, followed by (ii) administering a therapeutic candidate to the non-human mammal; and then (iii) determining a physiological effect of the therapeutic candidate in the non-human mammal.