The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.
申请公布号
EP2895618(A1)
申请公布日期
2015.07.22
申请号
EP20130762540
申请日期
2013.09.06
申请人
OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人
TURNER, DANIEL JOHN;BROWN, CLIVE GAVIN;REID, STUART WILLIAM;CLARKE, JAMES ANTHONY;WHITE, JAMES;STODDART, DAVID JACKSON