Stabilized subtilisin composition

摘要

The conversion of a peptide aldehyde to a hydrosulfite adduct can be used to increase the aqueous solubility in the purification of the peptide aldehydes. Advantageously, this hydrosulfite adduct is itself effective as a subtilisin inhibitor and stabilizer and it can also stabilize a second enzyme if present. The hydrosulfite adduct is effective as a subtilisin inhibitor, and it maintains its inhibitory and stabilizing effect in a liquid detergent during storage. Thus, use of the hydrosulfite adduct can avoid the cost and time of converting it back to the peptide aldehyde and subsequent drying of the peptide aldehyde can be saved, and this can avoid the inconvenience of handling the peptide aldehyde in powder form or as a highly diluted aqueous solution.

主权项

1. A composition, which comprises a subtilisin and a peptide aldehyde hydrosulfite adduct having the formula X—B1—NH—CHR—CHOH—SO3M, wherein:
a) M is H (hydrogen) or an alkali metal; b) R is a group such that NH—CHR—CO is an L or D-amino acid residue; c) B1 is one amino acid residue; and d) X consists of one or more amino acid residues, optionally comprising an N-terminal protection group.