Animal stroke model

摘要

An animal stroke model is provided. The model is useful in the study of brain ischemia and/or reperfusion injury and in identification and testing of compounds and interventions useful in the treatment of stroke. The method is carried out in a non-human mammal, such as rat. The method of inducing ischemia and/or reperfusion injury involves exposing a portion of the middle cerebral artery (MCA) and temporarily occluding it at one or more distinct locations, preferably three distinct locations. The model results in highly reproducible and focal infarct sizes, with low rate of mortality during the experimental procedure.

主权项

1. A method of inducing reproducible brain ischemia and optionally reperfusion injury in a non-human mammal, the method comprising:
exposing a portion of the middle cerebral artery (MCA) located in the area of the rhinal fissure and bifurcation of the MCA to the frontal and parietal cortices in the non-human mammal, temporarily occluding said MCA at a first location, a second location, and a third location to substantially occlude blood flow, wherein:
the first location is proximally dorsal to the rhinal fissure;the second location is proximally ventral to bifurcation of said MCA to the frontal and parietal cortices; andthe third location is proximal to bifurcation of said MCA along the parietal cortex; maintaining said occlusion for a time sufficient to induce a focal ischemia in the area of the MCA in the non-human mammal; and, optionally, releasing said occlusion to restore blood flow and maintaining said restored blood flow for a time sufficient to induce a reperfusion injury.