Fitases recombinantes, preparação de proteína, heterodímero, polipeptídeo imobilizado, arranjo, suplemento alimentar, matriz de liberação de enzima comestível e pélete comestível compreendendo as mesmas, bem como métodos de produção das referidas enzimas e para hidrolisar um inositol-hexafosfato, desgrudadura de óleo e processamento de grãos

摘要

A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.